Gaurav Pandey

PostDoctoral Researcher, gpandey@scripps.edu

I am a post-doctoral researcher in Prof. Mia Huang’s Lab in Department of Molecular Medicine at Scripps. Here, I am trying to utilize the combined potential of mass spectrometry-based glycoproteomics, protein engineering, and synthetic chemistry to decipher the role of proteoglycans and glycans in a myriad of pathophysiological pathways.
Previously, I was a postdoc in Prof. Ryan Julian’s Lab at UC Riverside, where I investigated the role of spontaneous chemical modification mediated lysosomal dysfunction in triggering neuronal loss and the potential effect of this phenomenon in the cascade of events leading to the disrupted proteostasis, amyloid formation, and tauopathies associated with Alzheimer’s Disease (AD).

Age-related diseases such as Alzheimer’s Disease (AD) at present cannot be prevented, cured, or even slowed. My doctoral research at IIT Guwahati was focused on the design, development, and optimization of cost-effective treatments of this mind-robbing protein aggregation mediated disorder. To achieve this, I employed a hybrid approach of utilizing non-invasive electric fields and structure-based designed peptides as chemical modulators. Our design approach for aggregation modulators has been in silico modeling techniques and molecular dynamics simulations, followed by experimental validation using state of the art biophysical and cell viability assays.

When I am not working, I do a lot of sport. I play badminton and tennis. I run and meditate. I love to spend time with my family. I also enjoy reading, learning about new things. Currently, I am trying my hands on graphic designing.


Research Interests

  • Mass Spectrometry
  • Glycobiology
  • Glycoproteomics
  • Small Molecule-Based Therapeutics
  • Autophagy
  • Proteopathies
  • Self-Assembly
  • Functional Biomaterials

  • Education

    Indian Institute of Technology, Guwahati, India

    Ph.D., in Biosciences & Bioengineering
    2013 - 2019

    University of Hyderabad, India

    Master of Technology, in Bioinformatics
    2011 - 2013

    Gautam Buddh Technical University, India

    Bachelor of Technology, in Biotechnology
    2007 - 2011

    Skills

    Computational Biology skills
    • Programming Languages: C, Python*, R*, Perl, LATEX. (*Elementary Profciency)
    • Databases: NCBI, RCSB, PDB-SUM, Swiss-Prot
    • Molecular Dynamics Simulation: Gromacs
    • Molecular Modelling Tools: Pymol, Avogadro, Chimera, Swiss-Modeller, Discovery Studio, VMD
    • Web Development: HTML
    • Others: Ribosome, Autodock

      Technical Skills
      • Biophysical Techniques: Spectroscopy (UV-Vis, Fluorescence, IR, CD, Raman), HPLC, Microscopy (AFM, TEM, FESEM), Powder XRD, DLS, ITC.
        Organic Synthesis: Solid Phase Peptide Synthesis.
        Mass Spectrometry: ESI-MS, nanoESI-MS,Gas-Phase Dissociation Techniques (CID, HCD, ETD, and UVPD), MALDI-TOF.

    Publications

  • Gaurav Pandey, and Ryan R Julian. LC-MS Reveals Isomeric Inhibition of Proteolysis by Lysosomal Cathepsins. Analysis & Sensing, 2022. DOI: 10.1002/anse.202200017
  • Gaurav Pandey, and Debika Datta. Peptide-Based Nanomaterials-Applications and Challenges. Book Chapter in De Novo Peptide Design: Principles and Applications, Elsevier , 2022.
  • Gaurav Pandey, and Vibin Ramakrishnan. Invasive and Non-Invasive Therapies for Alzheimer’s Disease and Other Amyloidosis. Biophysical Reviews, 2020.DOI: 10.1007/s12551-020-00752-y
  • Gaurav Pandey, Sudhir Morla, Sachin Kumar, and Vibin Ramakrishnan. Modulating Aβ Fibrillogenesis with ‘Trojan’ Peptides. Neuropeptides, 2020.DOI: 10.1016/j.npep.2020.102030
  • Gaurav Pandey, Sudhir Morla, Sachin Kumar, and Vibin Ramakrishnan. Modulating Tau Fibrillogenesis with ‘Trojan’ Peptides. Biochimica et Biophysica Acta - General Subjects, 2020. DOI: 10.1016/j.bbagen.2020.129569
  • Gaurav Pandey, Prem Prakash Das, and Vibin Ramakrishnan. Directive effect of chain length in modulating peptide nano-assemblies. Protein and Peptide Letters, 2020. DOI: 10.2174/0929866527666200224114627
  • Jahnu Saikia, Gaurav Pandey, Sajitha Sasidharan, Ferrin Antony, Harshal B. Nemade, Sachin Kumar, Nitin Chaudhary, and Vibin Ramakrishnan. Electric Field Disrupts Amyloid Aggregation; Potential Non-invasive Therapy for Alzheimer's Disease. ACS Chemical Neuroscience, 2019.DOI:10.1021/acschemneuro.8b00490
  • Gaurav Pandey, Sudhir Morla, Harshal B. Nemade, Sachin Kumar and Vibin Ramakrishnan. Modulation of Aggregation with Electric Field; Scientific Roadmap for a Potential Non-Invasive Therapy Against Tauopathies. RSC Advances, 2019. DOI:10.1039/C8RA09993F
  • Gaurav Pandey, and Vibin Ramakrishnan. Modulation of Aggregation with Electric Field; Electric feld assisted inhibition of aggregation in amyloidogenic proteins. Proceedings of the 35th European Peptide Symposium, 2018. DOI:10.17952/35EPS
  • Gaurav Pandey, Jahnu Saikia, Sajitha Sasidharan, Deep C. Joshi, Subhash Thota, Harshal B. Nemade, Nitin Chaudhary, and Vibin Ramakrishnan. Modulation of Peptide Based Nano-Assemblies with Electric and Magnetic Fields. Scientific Reports, 2017.DOI:10.1038/s41598-017-02609-z

  • Patents

    International Patent.
  • Patent No. WO/2019/012556. Dated 17.01.2019.
  • Title: A device treatment of neurodegenerative diseases.
    Patent No. WO/2019/012556

    Indian Patents
  • Patent No. TEMP/E-1/36478/2019-KOL. Dated 27.08.2019.
    Title: Peptide-based modulators of amyloidogenic peptides.

  • Patent No. 243/KOL/2015. Dated 09.03.2015.
    Title: Generation and usage of stimulus responsive nanostructures.

  • Patent No. 10258/2016-KOL. Dated 31.03.2016.
    Title: Magnetic hydrocarbon crystals.


  • Awards & Certifications

    • Received SCIEX Scholarship to Attend the Mass Spectrometry & Advances in the Clinical Lab (MSACL) LC-MS/MS 101 short course⁠.
    • Received International Travel Support from DST (Department of Science and Technology, India) to attend the 26th American Peptide Symposium held in Monterey, California, USA.
    • Awarded APS Travel Award to attend the 26th American Peptide Symposium held in Monterey, California, USA.
    • Awarded with First Prize in Oral presentation at International Conference on Advances in Biological Systems and Materials Science in NanoWorld 2017, IIT BHU.
    • Qualifed GATE 2013 with 99.45 percentile.
    • Secured rank 2 in M.Tech. Course at Department of Biotechnology and Bioinformatics, University of Hyderabad.
    • Qualifed GATE 2011 with 94 percentile.
    • Institute Award, for securing rank 1 in B.Tech. Course at Department of Biotechnology, CET-IILM-AHL.

    Curriculum Vitae